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Hi!

I am trying to reproduce the imaging conditions found in the following paper: Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Briefly: "The following settings have proven to work for MSPT on a commercial mass photometer (see Table of Materials): FOV of 128 pixels x 35 pixels, a frame rate of 1 kHz resulting in roughly 200 frames per second after subsequent 5-fold frame averaging, and an exposure time of 0.95 ms." ... "The movie duration can be set in advance and depends on the type of experiment. In most cases, an acquisition time between 5 min and 7 min is recommended."

We are using a OneMP with the latest software and I am having some difficulty modifying the frame rate/exposure time/acquisition time such that in one recording they are equivalent to those mentioned (ie. frame rate: 1 kHz; exposure time 0.95 ms; acquisition time 5-7 min). Is this…

Hello, I am starting a new project with a protein that has extensive intrinsically unstructured regions. What are the experiences of the community with this? I am searching for good references. I know it should be possible, but I am struggling to find literature references where authors did this. Thank you.

Hi, I'm having trouble creating a mass calibration when using slides coated in poly-L-lysine (for r-loop + protein interaction work). BSA, beta amylase, and thyroglobulin are sticking to the slides and I've had some success with beta gal. Are there any protein recommendations?

Hi, I'm having trouble creating a mass calibration when using slides coated in poly-L-lysine (for r-loop + protein interaction work). BSA, beta amylase, and thyroglobulin are sticking to the slides and I've had some success with beta gal. Are there any protein recommendations?

Hi,

I would like to export the mass data and the counts to re-fit the data using different software. How can I do that? Thank you very much,

My

Hi,

we recently got the hint that we could analyze our MP spectra with all the filters turned right down, to let in as much noise as possible (in both binding and unbinding). I saw that this was mentioned in the manual for the OneMP but we have a TwoMP with Discover MP v2024 R1. Is there a way to access this options in our setup too? Additionally, I found under preferences an advanced mode which requires a password that we are not aware of. Is there a way to get into the advanced mode?

Thank you for your help!

Axel

Why does the same sample give positive and negative peaks (see the attachment)? Does this result indicate binding and unbinding?

Thilini-Case Western Reserve

Is it possible to study interactions of synthetic nanoparticles with biological entities (e.g. formation of a protein corona around polystyrene nanoparticles) by mass photometry? I suppose calibration would be an issue, and perhaps there would be some other difficulties.

Thank you very much,

Jitka

Is it likely (currently or in future) that macro mass photometry would be used to characterize mycoplasma (or other bacteria) in some way? Some mycoplasma cells are around the upper size limit of KaritroMP but I understand that they are rather complex in comparison to viruses or biomolecules.

Thank you,

Jitka