Getting Started

Hi Ryan,

We have not tested “20 uL aliquots at 2X” and hence unable to comment on the product stability at such dilutions. But what we can say is for best results, aliquot 10 x 5 µL upon first thaw and store to avoid repeated freeze/thaw cycles.

Best,

Team Refeyn

Hello,

The mass precision (CoV) of the SamuxMP is 2%, meaning that is 4% difference in results is possible, but it is rare that it would be this large. There are a couple of things which can affect this (and the factors that can cause mass deviations for MFP2 are the same factors that can cause deviations for any other measurement):

1. Temperature: The temperature inside the system effects the contrasts and therefore the mass measured by the mass photometer. We would therefore recommend calibrating regularly (at least every 2 hours) and ensuring your system has been turned on for around an hour before use so it can reach thermal equilibrium.

2. Bubbles in immersion oil: Bubbles in the immersion oil can cause shifts in the measured mass. The latest versions of the software should detect these bubbles, but you can also look for breaks in the focus ring which can indicate a bubble in the oil. If you find one, it can be moved by removing the magnets and lifting the slide at one side enough to break the connection between the oil drop and the slide.

I hope this helps but please feel free to reach out again if you have any further questions.

Best regards,

Refeyn

Welcome to the Refeyn community! Total counts refers to the number of binding events plus unbinding events. Binding events refer to the number of particles that bound to the glass during the experiment, and an unbinding event is recorded if a particle that has landed on the glass happens to detach from the glass back into solution. The number of binding events in a properly run sample should be 1000-3000 depending on concentration and the type of sample you are running. We expect the binding events to be greater than unbinding events if the instrument is performing properly. If your sample is majority unbinding events that could indicate an issue with the focal position, slide, or buffer.

There aren't exact values for Brightness and Sharpness that are correct as these can vary depending on your instrument settings. The main thing you should look out for as the user is if the Sharpness and Brightness indicators in AcquireMP fall below their thresholds and turn orange while the instrument is in focus and a sample is on the slide. If you think you are experiencing issues with Sharpness or Brightness you can try emailing support.refeyn.com!!