Mass calibration with poly-L-lysine slides
Hi, I'm having trouble creating a mass calibration when using slides coated in poly-L-lysine (for r-loop + protein interaction work). BSA, beta amylase, and thyroglobulin are sticking to the slides and I've had some success with beta gal. Are there any protein recommendations?
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Hi Nicole,
Can I ask what you mean by sticking to the slides? We measure the particle signals as they bind to the slide. If you're not see individual particles during the recording, the concentration might be off. Try using 5-10 nM of protein for measurement
It might be easier to look at the data and provide better troubleshooting help. Feel free to email us at support@refeyn.com or to me directly at amy.chau@refeyn.com. We can discuss more in detail via email or call.
Amy Chau
Sr. Field Applications Scientist