What affects MassFerence P2 Calibration?
Hi, what affects the % error for the massference P2 calibrants? I tried doing 2 calibrants for a slide and the % error is around 4%.
i would appreciate a response. Thank you!
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Hello,
The mass precision (CoV) of the SamuxMP is 2%, meaning that is 4% difference in results is possible, but it is rare that it would be this large. There are a couple of things which can affect this (and the factors that can cause mass deviations for MFP2 are the same factors that can cause deviations for any other measurement):
Temperature: The temperature inside the system effects the contrasts and therefore the mass measured by the mass photometer. We would therefore recommend calibrating regularly (at least every 2 hours) and ensuring your system has been turned on for around an hour before use so it can reach thermal equilibrium.
Bubbles in immersion oil: Bubbles in the immersion oil can cause shifts in the measured mass. The latest versions of the software should detect these bubbles, but you can also look for breaks in the focus ring which can indicate a bubble in the oil. If you find one, it can be moved by removing the magnets and lifting the slide at one side enough to break the connection between the oil drop and the slide.
I hope this helps but please feel free to reach out again if you have any further questions.
Best regards,
Refeyn