Modification of imaging conditions
Hi!
I am trying to reproduce the imaging conditions found in the following paper: Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
Briefly: "The following settings have proven to work for MSPT on a commercial mass photometer (see Table of Materials): FOV of 128 pixels x 35 pixels, a frame rate of 1 kHz resulting in roughly 200 frames per second after subsequent 5-fold frame averaging, and an exposure time of 0.95 ms." ... "The movie duration can be set in advance and depends on the type of experiment. In most cases, an acquisition time between 5 min and 7 min is recommended."
We are using a OneMP with the latest software and I am having some difficulty modifying the frame rate/exposure time/acquisition time such that in one recording they are equivalent to those mentioned (ie. frame rate: 1 kHz; exposure time 0.95 ms; acquisition time 5-7 min). Is this…
Hi Cecilia,
Mohammed is our support person for software.
You can modify the Photometer Settings from the Settings menu, at the top left of AcquireMP; here you have the field of view, frame rate and frame averaging – check the user manual for further details. You might need to activate Advanced Mode first, from the Preferences menu. If you are trying to reproduce the results of the paper you mentioned, I would also recommend you unselect Compress Movies from the Preferences menu, to enable custom movie analysis with your own scripts. As for the recording time, you can change the number of seconds from the text box right below the movie, in AcquireMP’s main window.
The settings described should be achievable with your setup, but do not hesitate to contact our support@refeyn.com service if needed.
Kind regards,
Refeyn Team