Is there a way to subtract buffer background?
I'm trying to look at oligomerization for a 37kDa protein, and there is a lot of overlap between the monomer and the background from the buffer. Is there a way to subtract the background from the monomer peak?
Thanks.
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Hi Felicity,
No, there is no way to do that. If they are using MassGlassUC we would recommend they hand clean the slides for any protein smaller than 50 kDa (protocol attached), and that they make sure they use a buffer without noise. They could use 1x buffer for cell culture grade, or if the buffer is prepared from tablets or concentrates they could mix it for a long time at a temperature higher than room temperature to favour a better dissolution of all components.
Best Regards,
Team Refeyn