export data from discoveryMP
Hi,
I would like to export the mass data and the counts to re-fit the data using different software. How can I do that? Thank you very much,
My
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Filter settings
Hi,
we recently got the hint that we could analyze our MP spectra with all the filters turned right down, to let in as much noise as possible (in both binding and unbinding). I saw that this was mentioned in the manual for the OneMP but we have a TwoMP with Discover MP v2024 R1. Is there a way to access this options in our setup too? Additionally, I found under preferences an advanced mode which requires a password that we are not aware of. Is there a way to get into the advanced mode?
Thank you for your help!
Axel
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Positive and negative peaks for the same sample
Why does the same sample give positive and negative peaks (see the attachment)? Does this result indicate binding and unbinding?
Thilini-Case Western Reserve
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Is there a way to subtract buffer background?
I'm trying to look at oligomerization for a 37kDa protein, and there is a lot of overlap between the monomer and the background from the buffer. Is there a way to subtract the background from the monomer peak?
Thanks.
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Team Refeyn
Jul 21, 2025
Hi Felicity,
No, there is no way to do that. If they are using MassGlassUC we would recommend they hand clean the slides for any protein smaller than 50 kDa (protocol attached), and that they make sure they use a buffer without noise. They could use 1x buffer for cell culture grade, or if the buffer is prepared from tablets or concentrates they could mix it for a long time at a temperature higher than room temperature to favour a better dissolution of all components.
Best Regards,
Team Refeyn
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Hi My Le,
Yes, if you have version v2025 R1 (or later) of the software installed you can go ahead and update. Please contact support@refeyn.com if you have any further questions or issues.
Best regards,
Team Refeyn