Is it possible to study interactions of synthetic nanoparticles with biological entities (e.g. formation of a protein corona around polystyrene nanoparticles) by mass photometry? I suppose calibration would be an issue, and perhaps there would be some other difficulties.
Thank you very much,
Jitka
Is it likely (currently or in future) that macro mass photometry would be used to characterize mycoplasma (or other bacteria) in some way? Some mycoplasma cells are around the upper size limit of KaritroMP but I understand that they are rather complex in comparison to viruses or biomolecules.
Thank you,
Jitka
I had a question regarding the limit of detection using the MassFludix device. I read that the lower limit is 50 kDa. My protein of interest is ~35 kDa based on MP measurements and SEC analysis. If I were to run this protein on the MassFluidix what would happen? I noticed that running a PBS blank produces a peak that was called ~54 kDa, close to the limit of detection. For a protein below the 50 kDa cutoff would it look similar to a PBS run?
Hello Refeyn Team,
In the case of a protein complex where the affinities are not known, or are beyond the permissible range of the instrument (say, for instance, in the uM range), could I make a reaction volume at a high enough concentration, allow that to incubate, and then make a dilution of that complex to the concentration range that I typically use for finally adding to the cover slip?
Thanks a lot, and I look forward to it.
Warmly,
Ayush