Measurement Standards Query
Hi folks at Refeyn, I wanted to know if I can use beta-amylase (which mass calibrates 56, 112, and 224 kDa) to mass calibrate for capturing a protein that oligomerizes to ~500 kDa. Thanks a lot, and I look forward to hearing from you folks.
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Hi Ayush,
A calibration with beta-amylase has a very good linearity in that range, but if you want to be sure you can always add a higher mass protein like thyroglobulin at 670 kDa.
Best wishes,
Team Refeyn