What is the location accuracy of the landing event detection?
Our location accuracy goes down to about 1 nm - but this depends on the mass of the particle. For proteins, we usually observe about 10 nm at 40 kDa and 1 nm at 400 kDa.
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Hi Jason,
You are right that the pixel resolution of the camera image is worse. Each pixel (after binning) represents a distance of approximately 84nm. However, when we fit the Point Spread Function to the image, we obtain the event location with a sub-pixel accuracy. The reason it varies, from approximately 1nm at 400kDa to 10nm at 40kDa, is due to the reliability of the fit. As the mass of the particle is reduced and we get near to the detection limit, the event becomes more dominated by noise. This relative increase in noise reduces the accuracy of the Point Spread Function fit. With a less accurate fit, the accuracy of the event location will be lower.
Hope this helps!