Counts vs Binding Events
Hello, I'm new to this technology.
I wanted to know what the differences are between the counts and the binding events.
In the quality metrics section of the report, I see binding and nonbinding events. What are the acceptable numbers for these? And also, for the Mean Sharpness and the Mean Brightness? What is considered acceptable for these quality metrics?
Thank you
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Welcome to the Refeyn community! Total counts refers to the number of binding events plus unbinding events. Binding events refer to the number of particles that bound to the glass during the experiment, and an unbinding event is recorded if a particle that has landed on the glass happens to detach from the glass back into solution. The number of binding events in a properly run sample should be 1000-3000 depending on concentration and the type of sample you are running. We expect the binding events to be greater than unbinding events if the instrument is performing properly. If your sample is majority unbinding events that could indicate an issue with the focal position, slide, or buffer.
There aren't exact values for Brightness and Sharpness that are correct as these can vary depending on your instrument settings. The main thing you should look out for as the user is if the Sharpness and Brightness indicators in AcquireMP fall below their thresholds and turn orange while the instrument is in focus and a sample is on the slide. If you think you are experiencing issues with Sharpness or Brightness you can try emailing support.refeyn.com!!