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Refractive Index affected by salts

When I look at samples with elevated salt concentrations, like MgCl2 for example, I see what looks like TV static in the background. I see from a quick search that the salt is likely affecting the landing behavior and the refractive index contrast.


I'm curious if anyone has done a study of different salts at different concentrations that I could look to for guidance when doing sample prep? I don't want to burn through the consumables on something like that.


Thanks in advance for any help or insights!

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Austin Graves
5 days ago

Hi Mike,

Is your buffer just MgCl2 or does is it added to PBS? We have observed background noise coming from magnesium but this often occurs when the MgCl2 buffer is prepared in PBS.


At the moment we do not have a document that outlines the possible effects of different buffer components but I will certainly carry that feedback back to the team. Thanks!


Austin G.

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