TwoMP Autofocus Failure: Causes and Troubleshooting
What are the common reasons why the TwoMP fails to find focus in droplet-dilution mode, and how can I prevent this?
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Troubleshooting
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Mass calibration with poly-L-lysine slides
Hi, I'm having trouble creating a mass calibration when using slides coated in poly-L-lysine (for r-loop + protein interaction work). BSA, beta amylase, and thyroglobulin are sticking to the slides and I've had some success with beta gal. Are there any protein recommendations?
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Team Refeyn
Feb 27
Hi Nicole,
Our technical support representative will get in touch with you.
Kind regards,
Team Refeyn
We get different number of counts even with the same concentration of protein samples between different measurements, why is that?
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Brenda Watt
Feb 26
As soon as sample is added and mixed, molecules start landing on the glass slide. Variability in pipetting, mixing, and starting the recording will all contribute to variations in counts, even if starting with the same sample concentration. Most landing events occur right after adding sample and will decrease over the course of the measurement, so trying to keep an even pace (same number of pipette mixes up and down, same time between mixing, closing the lid and hitting record will all help keep your counts more reproducible between measurements.
Negative molecular mass
Why am I seeing very high negative molecular mass histograms? Mass calibration seems ok and worked well for previous measurements.
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Hi Ying,
There are a couple of possible reasons why this could be happening but we would need more information to be able to diagnose it. Please reach out to support@refeyn.com with your issue and we can connect you with your local representative who can help figure out what's going on.