White events in Ratiometric view
A few times in recent experiments, I have noticed the first few readings have come up with negative mass values for my protein of interest (Events in ratiometric view appear as white dots). Eventually, using the same sample of protein and technique, the machine begins displaying a positive mass value and black event dots. Any thoughts as to why this occurs? I am sure to warm up the machine for 30 minutes before beginning. I have attached photos as to what the ratiometic view shows.
Another observation is that the negative mass values when this occurs are not reflections of the positive values. When the mass is negative, it is usually around -180 kDa, while when positive it is around 220 kDa.
As always, thank you for your time and help!
180 Views
I am a new user of Mass Photometer. I was wondering that why do we see peaks around 0 and -ve molecular weights? Also, why do we see reflective peaks in negative ( they correspond to the molecular mass in positive). I would be thankful if anyone can explain this to me. Looking forward.