Software

AcquireMP saves measurement data as MP files (*.mp). MP files do not only contain the frame data but also all the acquisition settings. MP files can be opened with DiscoverMP for analysis. MP files can also be read by using widely used applications and libraries (Matlab, IGOR Pro, pandas, etc.) because the files follow the HDF5 is an open-source file format. Note: if you want to read your files with another application than DiscoverMP you are advised to disable file compression under Preferences in AcquireMP. Otherwise the compressed raw frame data will be difficult to interpret.

Acquisition settings of AcquireMP and analysis settings of DiscoverMP are stored as JSON files (*.json). In principle, those files can be opened with any text editor. However, we do not recommend editing these files manually. Instead use these files from within AcquireMP and DiscoverMP to save or restore your settings.

DiscoverMP saves analysis results together with a copy of the measurement data (named “movie.mp”) into results folders. CSV files can be opened with Microsoft Excel or with any text editor, while H5 files can be opened with programs that can read HDF5 files (see above).

In addition to the measurement data the following files can be found in the results folder:

• Analysis settings: settings.json

• Event information (peak contrast, mass, frame index, position coordinates, etc.)

  • eventsFitted.csv/.h5: event information after peak fitting

  • eventsFound.csv/.h5: event information before peak fitting (only for internal use)

• Peak thumbnails from the ratiometric images: peaks.h5

• Ratiometric frames: ratiometric.h5

• Quality score traces (sharpness, motion, saturation, signal,…): scores.h5

• Mass calibration (data and calibration parameter values): *.mc

• Application meta information for version, warnings, selections and annotations: overlays.json and movieQuality.info