Coating glass coverslips with Poly-L-Lysine (PLL)
Can poly_D_lysine be used instead of poly_L_lysine? Should these slides be freshly made, or can they be stored for long?
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Mass Photometry Filter
What is the best filter to use for filtering the buffer? I have tried a 0.22 um syringe filter but it was adding 2500 counts around 50 kDa. The filters are sealed and not expired. Is there a specific brand recommended?
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Ready-to-use slides not working properly
We have purchased some ready-to-use slides, but every time we try to use them they are much more dirty than normal slides. Wonder if it happens with all these slides. If so, information is not clear about them.
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Chase
30 ene 2024
You may have gotten a bad lot. I have generally had good experience with pre-cleaned slides and find them to be more consistent than the self-cleaned slides I've been prepping for a few years. Be sure to keep partially used 10-packs taped in their original foil packaging as they tend to attract dust from the room air pretty quickly. However, if you are finding the slides are dirty even right out of the package, I'd document and ask for a replacement. For the price, they should be working for you.
14 sample wells/cassette
Hello, is it possible to use the "14 sample wells/cassette" on the non automated TwoMP?
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Wesley Jackson, Jr
31 may 2023
To Team Refeyn,
Can you expand on the reason why the larger sample well cassettes can't be used on the TwoMP? I have not tried yet but if we are independently moving the stage to the wells I couldn't see why the cassettes with more wells can't be used. Even if you can only use say 6 of the wells because the slide can only be moved so much. Just curious.
Best.
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Hi Emma,
We recommend paying attention to the filter composition, as some filters are known to introduce small quantities of surfactants in the solution that might create noise in mass photometry.
We have these syringe filters internally which we are happy with: gb.gilson.com/syringe-filters-pvdf.html
Another point would be that contamination in the 50kDa range is unlikely to be removed by a 0.22um filter as I would imagine anything that low in mass could pass through the filter.
Hope that helps!
Best,
Team Refeyn