Do detergent molecules that surround the protein of interest affect the mass determined by mass photometry?
Yes, they do. Since mass photometry determines the contrast of the entire complex that binds to the glass surface, its detergent belt is included in the total mass of the particle.
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The new application note about the use of detergents in mass photometry is now available for download.
Check it for a more ellaborated explanation on this as well as examples and a useful table of expected noise level for a number of common detergents and concentrations to guide your experimental design.