Buffer background
Hi,
I am trying to analyze oligomeric state of my protein (blue) but the signal of the monomer (-0.02) is overlaping with some noise from the buffer (orange). Moreover, I can see that some of the events appear in the positive contrast as well. What is the proper way of treating the data to extract the counts for individual species?
Thank you
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The chances to get information about protein status when the signal is overlapping with noise are low. Concentration increase could be a solution.