What happens when the particle concentration is too high?
The analysis of mass photometry data at high concentrations becomes challenging because of signal overlap. As particle concentration increases we usually observe:
a lower fraction of successfully identified peaks (overlapping peaks have a different signature than single peaks and can therefore not be distinguished and detected individually)
a lower mass precision and hence peak broadening in the mass histogram and decreased resolution.
As a rule of thumb, the total number of events should not be higher than half the number of frames, ie. we recommend less than 3,000 events for a video of 6,000 frames.
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