Do fluorophores and chromogenic compounds affect measurements?
Fluorophores that emit in the green range of the spectrum (e.g. YFP, CY3) might interfere with the data because they emit similar wavelengths to the light collected for the measurements. In addition, fluorophores that are excited in the green might promote photodamage to the sample. On this page there is a list of fluorochromes used in microscopy with emission profiles similar to YFP:
https://www.microscopyu.com/techniques/fluorescence/nikon-fluorescence-filter-sets/yellow-fluorescent-protein-yfp-excitation
Fluorophores with an emission profile that covers the red range of the spectrum might interfere with the auto-focus of the mass photometer. We have observed that A555 affected the diameter of the autofocus ring.
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