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Regarding Weak Binding Affinities

Hello Refeyn Team,


In the case of a protein complex where the affinities are not known, or are beyond the permissible range of the instrument (say, for instance, in the uM range), could I make a reaction volume at a high enough concentration, allow that to incubate, and then make a dilution of that complex to the concentration range that I typically use for finally adding to the cover slip?


Thanks a lot, and I look forward to it.


Warmly,

Ayush

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